Inhibitory Effects of Red Grape Seed Extracts on Pancreatic -amylase and Lipase

The purpose of present study was to investigate the effect of water and ethanol extracts of red grape seeds on inhibition of pancreatic -amylase and lipase in vitro. Total proanthocyanidins were determined in grape seed extracts (GSEs). Different concentrations (400, 800, 1300 and 1800 ppm) of each GSE were employed to evaluate their potentials on pancreatic -amylase and lipase after pre-incubation with enzyme or substrate. The obtained results showed that ethanol grape seed extract contains higher concentration of proanthocyanidins (32.64 mg/g grape seeds) compared with water grape seed extract (26.72 mg/g grape seeds). Data also showed that the GSEs possessed inhibitory effects against some pancreatic enzymes (i.e. pancreatic -amylase and lipase). Inhibitory activity of each GSE is occurred either pre-incubation with enzyme or substrate. There was a positive correlation between GSE concentration and inhibitory activity. The ethanol grape seed extract (EGSE) had higher inhibitory effect against pancreatic -amylase and lipase in comparison with water grape seed extract (WGSE). In conclusion, the GSEs rich in compounds that inhibit -amylase and lipase may provide a safe, natural and cost-effective in the treatment of diabetes and obesity.